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Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward [14]
After 20 min of derivatization reaction to stop the process 50 L of 3 mol/L PCA was added and 10 L of the final solution was transferred into HPLC column
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doi: 10.1016/j.cell.2012.03.042 18 Brigelius-FloheRMaiorinoM
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