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The pellet was re-suspended in 12 mL lysis buffer (25 mM HEPES p H 7.5, 100 mM NaCl, 5% (w/v) glycerol, 5 mM GSH)

Short-term safety, tolerability and efficacy of a very low-calorie-ketogenic diet interventional weight loss program versus hypocaloric diet in patients with type 2 diabetes mellitus

Your choice should prioritize personal convenience and side-effect tolerance rather than efficacy differences

This consistency is crucial for researchers to meet experimental timelines and have continuous access to research materials