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After tipping off the supernatant, plates were washed, blotted on paper and blocked with PBS 1% BSA for 30 min at RT

There is preclinical and animal data along with methodologically flawed studies

By inhibiting NNMT, 5-Amino-1MQ increases nicotinamide pool availability (NAD+ precursors), elevates cellular SAM supporting methylation reactions, reduces adipogenesis, and enhances energy expenditure through improved mitochondrial function

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