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a,b , Representation of the stationary points calculated with ONIOM(B97X-D/6-311 + G(2d,p):ff14SB,gaff2,TIP3P) G(d):ff14SB,gaff2,TIP3P) for the Pt GSTF8-catalysed deprotonation-protonation reaction: flavan-3-on-4-ol ( 7 ), transition state for deprotonation ( 7-TS ), deprotonation product ( 8-enolate ) and cyanidin ( 8-B4 ) bound to the wild-type enzyme ( a ) and the C13S variant ( b )

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Thus, the residual absorbance only represents unreacted substrates, providing a contamination-free result

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10.3389/fnins.2020.00213 Front