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[47]Zhu S, Shi W, et al
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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C
The result is often exactly the problems copper peptides are supposed to solve: irritation, premature aging signs from inflammation, and compromised skin barrier function
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