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To assess how the loss of ATP6AP1 KO affected acidic compartments, we used the hydrophobic dye LysoTracker Red DND-99, which in acidic environments becomes rapidly protonated, predominantly labeling late endosomes and lysosomes
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And perhaps most notably, we anticipate FDA will seek to understand lessons learned from the pandemic and how to apply those lessons moving forward, including how to best leverage alternatives to on-site inspections, such as 704(a)(4) records requests, voluntary remote regulatory assessments, mutual reliance on inspectional findings from foreign authorities, product sampling and testing, and other tools