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Activated G-proteins may stimulate the production of secondary messengers such as cyclic adenosine monophosphate (cAMP) and inositol trisphosphate (IP3)
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Cells were first gently lysed with the cytoplasmic extraction buffer to release cytoplasmic components, and the supernatant was collected after centrifugation as the cytoplasmic protein fraction
[13] In GPX1 (-/-) mice, oxidant formation is increased, endothelial NO synthase is deregulated, and adhesion of leukocytes to cultured endothelial cells is increased
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