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In addition to systemic benefits, as early as 1940, it was observed that riboflavin could also have local benefits: vaginal administration of riboflavin in ten women via a lactose tablet was shown to result in an increased acidity and a favorable impact on vaginal bacteria through increased growth of acid-forming bacilli and decrease of pathogens 78

In the UK, the standard NHS regimen for B12 deficiency without neurological involvement is hydroxocobalamin 1mg IM three times a week for two weeks