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In the day of the experiment, cells were stained with 10 M DCFH-DA at 37 C for 30 min, then were treated as described above for 4 h
Since NAD + is required for ATP production, FK866-induced NAMPT inhibition affects ATP production and, consequently, results in AMPK activation (in hepatocarcinoma cells)
Membranes were blocked with 5% BSA in tris-buffered saline (TBS) with 0.1% tween (TBS-T)
& Anand, S
These drugs can support weight loss and improve insulin sensitivity, which are two big challenges for many Cysters
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