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N237S) disorder characterized by an increase in reactive oxygen species (ROS), chronic loss of axons and progressive loss of nervous system function in pediatric patients

TMAO can also promote endoplasmic reticulum stress (ERS) by up-regulating SR-1 receptors, while downregulating ATP-binding cassette transporter A1 in macrophages (64)

Other standard reagents used in cell culture experiments included DMEM basal medium, fetal bovine serum, and a penicillin-streptomycin antibiotic mixture, as well as dimethyl sulfoxide (DMSO) and the Cell Counting Kit-8 (CCK-8), which was used to measure cell proliferation

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