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39 For immunohistochemistry, the tissue sections were sequentially placed in xylene I for 15 mins, xylene II for 15 mins, anhydrous ethanol I for 5 mins, anhydrous ethanol II for 5 mins, 85% alcohol for 5 mins, and 75% alcohol for 5 mins, and then were finally washed using distilled water

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Published studies in animal models have reported effects on fibroblast activity the cells responsible for producing collagen and structural proteins as well as collagen organization and tissue remodeling signals

MOTS-c mitochondrial-derived peptide cross-referenced in metabolic-rate research