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For LDH assays, 60 l of supernatant from wells containing BMDMs was collected into 96-well plates and LDH assay buffer was added according to manufacturers instructions (Promega CytoTox 96 Non-Radioactive Cytotoxicity Assay, G1780)

Together, these findings suggest a diversion of glucose flux away from glycolysis at this critical branch point in the pathway

Like many therapeutic peptides, epitalon is typically cycled, which means using the drug for a specific period and then taking a break from it