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Iron injection restores brain iron and hemoglobin deficits in perinatal copper-deficient rats

After the reaction was complete, 48 kg of isopropyl acetate at room temperature was added to the reactor and the resulting mixture was maintained for 1 hour at 43-48 with moderate agitation, followed by filtration of the reaction mixture to remove the solids (Tyvek filter, 15.5" diameter, Kavon Filter Products, Wall, NJ, model No

Slides were counterstained with DAPI and coverslipped with Prolong TM Gold Antifade mountant (Thermo Fisher Scientific)