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Common impurities to check include truncated peptide sequences (Gly-His or His-Lys fragments), residual coupling reagents and protecting groups, counterion residues, free (uncomplexed) GHK, and excess copper salts

Peptides often interfere with endotoxin detection due to their tendency to bind or mask endotoxins, which can lead to inaccurate low results

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In GHK-Cu, it denotes that the tripeptide glycyl-L-histidyl-L-lysine (GHK) is bound to a copper(II) ion, forming the copper-peptide complex researchers study the Cu specifically distinguishes the copper-bound complex from the standalone GHK peptide, which research has shown behaves differently without the copper ion attached

The rubber stopper should not be removed from the vial, as this compromises sterility and could lead to contamination