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However, the presence of H3K4me3 does not predict efficient gene expression as the majority of inactive genes experience transcription initiation (Guenther et al., 2007)

doi: 10.1007/BF01225454 2 CreutzfeldtW

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The MBP flow-through was then subjected to GST resin, washed with wash buffer containing 2 mM ATP, and bound proteins were eluted with wash buffer containing 50 mM reduced glutathione