NADH is thought to stimulate endogenous L-DOPA (might increase tyrosine hydroxylase activity)
To test whether -KG insufficiency results in NADPH depletion, we used genetically encoded fluorescent sensors to monitor cytosolic (iNap1) and mitochondrial (iNap3) NADPH levels, as described previously 29
10993-1-AP, China), or anti--tubulin (1:5000, Huaxingbio, cat
Abbreviations GSH: Glutathione TTH: Tension-type headache HC: Healthy control MRS: Magnetic resonance spectroscopy MEGA-PRESS: Mescher-Garwood Point Resolved Spectroscopy ROC: Receiver operating characteristic AUC: Area under the curve MIDAS: Migraine Disability Assessment HIT-6: Headache Impact Test-6 CRLB: Cramr-Rao lower bounds FWHM: Full width at half maximum Glu: Glutamate mI: Myo-inositol tCho: Total choline NAA: N-acetylaspartate tNAA: Total NAA ROS: Reactive oxygen species NAC: N-acetylcysteine References Steiner TJ, Stovner LJ, Jensen R, Uluduz D, Katsarava Z (2020) Migraine remains second among the worlds causes of disability, and first among young women: findings from GBD2019
Protein Cell 15 , 419440 (2024)