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The severity depends on several factors: speed of weight loss, baseline protein intake, micronutrient status, and individual genetic predisposition
For ELISA, liver tissues were sonicated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and protease inhibitor cocktail (EDTA-free Complete Mini, Roche)
Zheng Z, Zhao K, Yang S
[ [72] Liu H, Liu Y, Jin SG, et al
Redox changes precede the occurrence of oxidative stress in eyes and aorta, but not in kidneys of diabetic rats
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