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siRNA transfections were performed using Lipofectamine 2000 and ON-TARGETplus Human SMARTpool siRNAs (Dharmacon) against TYMS and a scrambled control pool, according to the manufacturers instructions
The study didnt test if more alcohol led to further decreases in IGF-1
A 2025 systematic review identified 36 injury-recovery studies, but 35 were animal studies and only 1 involved humans, with no large randomized controlled trials available
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For characterization of cARLA-treated hECs the cells were cultured in 50% PC-conditioned medium on glass bottom chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific) coated with collagen IV and fibronectin at a seeding density of 5 10 4 cells/cm 2
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