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Adjuvant cox inhibition augments sting signaling and cytolytic T cell infiltration in irradiated 4t1 tumors

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Cell pellets were resupended in ice-cold buffer 1 containing 20 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C and stirred on ice with DNase I and 10 mg/mL lysozyme for 1 h before sonication

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The suppression of the type I IFN response by ROS is due to oxidation of cysteine 147 of murine stimulator of IFN, an ER membrane protein that mediates the IFN response after cytoplasmic DNA sensing 59,133,134